Miticide resistance - the facts
Extracts from Bee-L
The following exchange was on bee-l about
fluvalinate resistance (e.g. Apistan resistance). The
initial exchange is a bit heated due to a "glib"
response, for which Allen appologises.
Max Watkins
email@omitted.anti.spam
Allen,
In a recent communication about the evolution and
spread of pyrethroid resistance in Europe the following
comment was made:
It is thought that beekeepers in Italy used a
liquid agricultural formulation of Klartan, absorbed on a
porous piece of wood or similar to combat varroa. Because
there was no control over the amount (of active
ingredient) applied, this practise inevitably gave rise
to resistance, not only to Klartan, but to the whole
class of synthetic pyrethroids including Bayvarol and
Apistan.
You replied:
This is often repeated, but is at best an
oversimplification, and at worst a lie that those who
sell a penny's worth of chemical for $ don't mind having
repeated often.
That is a glib response, offensive not only to me but
to the well-respected bee researchers from Germany,
Italy, Switzerland and France as well as the hundreds of
beekeepers who carried out the trials which proved this
to be the case. Research such as this cannot be dismissed
because there was some involvement from a chemical
company (Sandoz, as it was). Especially when you don't
seem to know or care about the reality of this situation
- even 'though I have posted this on the LIST some time
ago. I am really surprised at you.
The statements which John Burgess mentioned originally
are far from rhetoric from a "chemical company". There is
solid foundation to these claims.
In the early 90's as the then Technical Manager for
Sandoz I coordinated an international team of
independent, reknown experts on a project lasting 5
years, investigating the emergence of pyrethroid
resistance in Europe.
It had been openly stated by many people, incuding
Sandoz, way before the conception of APISTAN that a
pyrethroid-resistant mite strain would inevitably evolve.
Klartan/Mavrik was being used for years before APISTAN
was developed.
Providing there is a large enough selection pressure
and the benefits outweigh the costs, organisms mutate
through natural selection. FACT. Any and all treatments
to control "pest" populations represent a type of
selection pressure. FACT. Because of its many benefits of
bee safety, low residue profile, proven low mammalian
toxicity profile etc etc Apistan (or illegally
Klartan/Mavrik) was and indeed still is the most popular
hive treatment for varroa worldwide. Incredible selection
pressure. FACT.
When the first reports of "Apistan inefficacy" arose
in 1992 in Italy, APISTAN had only been in use for one
year. In the regions where the "inefficacy was most
widely reported APISTAN was not used at all; the
agrochemical Klartan, however, had been used for 8 years,
on balsa wood, on cloth and anything else to hand.
Recipes for using Klartan were common in Europe. In
Spain they recommended a 5% soltion in water; in France
it was 2% solution. This didn't work in Southern Italy
after a while and so the dose was increased to 50% and
then in many cases, to 100% neat. Low efficacy after 8
years. FACT.
When this inefficacy started to show up in Northern
Italy, where APISTAN had been used for just 2 years the
cause was not so clear. However, the Italians were able
to trace exactly the spread of a resistant strain of
varroa along the main routes of migratory beekeeping from
the North to the South of Italy. Colonies are taken to
the South for the winter and brought back North in
Spring, carrying with them the resistant varroa from the
South. FACT.
It is no one's fault that resistant varroa emerged, it
is a natural process of evolution. The uncontrolled
dosing did however greatly speed up the process. If there
is an increase in the LD50 (the level of a substance that
wil kill 50% of a population) by tenfold, ie a resistance
factor of 10, generally one can say that a resistant
strain has emerged. In the case of varroa from Southern
Italy the resistance factor was more than 400. Definately
resistant. It was also shown scientifically that this
resistance is conferred to other related pyrethroids such
as flumethrin and acrinathrin. FACT.
The dose/response curves obtained for this resistant
strain are distinct. Wherever resistance was monitored
throughout Italy and later in France, Germany,
Switzerland, Austria and Eastern Europe. the exact same
curves are characteristic. This most probably means that
the resistance has arisen only once in Europe and spread
from one focal point. The slow speed of apparition in
neighbouring countries suggests Italy to be the focal
point. If the dose/response curves were markedly
different for the different populations examined then we
could expect there to have been separate evolution. So
far all populations examined (coordinated by Vita since
1997) have shown the same pattern and seem then to have
the same origin.
There will always be a risk of any pest organism
developing resistance to the treatment. It is why several
different types of treatment should be used. If there is
resistance to one treatment, perhaps the second or third
[different treatment will take out the resistant
individuals. Even IPM will not stop the phenomenon of
mutation and evolution in pest populations but the aim is
to keep the resistance to a manageable level, below the
economic damage threshold.
Vita (Europe) Limited is a small UK company
specialising in the development of honeybee disease
treatments. We take the concept of IPM very seriously and
unlike the chemical giants who have no time for such
small markets, we are dedicated to improving the health
of honeybee colonies, operating world-wide. Yes, we make
and sell APISTAN but we also have other treatments in
registration and in development, many of them natural
agents, for EFB, AFB, mites, chalkbrood and wax moth.
In science and in business I believe there is no room
for half-truths. At Vita our policy is to be open so as
to avoid confusion. While many people may disagree with
what we're doing, Vita is not in the business of making a
fast buck. What we do is thoroughly researched and is for
the long-term. No short cuts, no "oversimplifications".
Whatever is posted to the LIST from Vita, we believe to
be correct and true.
But, Hey, how can this possibly be true - Vita must be
classed as a "chemical company", right?
Believe what you will.
Max
Dr Max Watkins
Vita (Europe) Limited
Brook House, Alencon Link
Basingstoke, Hants RG21 7RD UK
http://www.vita.demon.co.uk
Allen Dick
email@omitted.anti.spam
Max, I want to apologise if I have offended you or any
others. That was certainly not my intent, and maybe the
writing was a bit glib. I sometimes kid around a bit.
I certainly respect the efforts you and many others
have put into fighting varroa and in no way discount the
work because some of it was done with funding by a
chemical company. I respect the profit motive and think
it is an important engine of development.
Max, I will also confess right now to having a
less-than-perfect memory and to being influenced by the
many versions of the story I have heard, especially since
they are repeated so often. I will have to review the
material and perhaps revise my thinking when I have a
moment. Once again, I should likely reiterate that this
is a discussion list and what is written here -- by
anyone -- should be questioned and not mistaken for
carefully researched fact. Maybe your contributions are
different, but I know the above is true of what I write
and would be very concerned if anyone swallowed any of my
opinion pieces whole.
Having said that, and although I am sure that you are
probably as authoritative as any man alive on the topic,
that does not mean that your view or your version, or
your conclusions, or your methods are the only ones that
are credible.
As evidence, I can offer that varroa is still causing
havoc. We still can see a day coming soon when we may
very well have no effective and practical controls
available. That to me proves that the job is not done, or
was not done well. I'm sorry, but that is just the way it
seems to me. I hope you can and will prove me wrong.
Once again I apologise and hope you can show us that
the problem was handled correctly, is under control, and
will stay that way.
Allen
Lipscomb, Al
email@omitted.anti.spam
I get a little confused on these evolution things. I
always thought that "mutation" was a random event.
Pressure then caused selection on the population. If the
mutation gave a reproductive advantage then those that
had the gene may survive and reproduce better than the
ones without the gene.
If putting pressure on a population always causes it
to become resistant I do not understand how we got
smallpox under control. You would have thought that
resistance would have emerged and a stronger version of
the virus taken over.
Come to think of it I cannot understand how extinction
should happen as often as it does. In most cases it is a
long slow process with lots of pressure on the
populations.
Al
Blane White
email@omitted.anti.spam
Hi Al and Everyone,
No wonder you are confused the term "evolution" used
in this context is just plain wrong and confusing. The
correct term is adaption. Resistance is always present in
the population at very low levels and the selection
pressure of the treatment brings it to the fore. No
mutation needed just selection. The result is a resistant
population.
Now Max, from what it appears to me here in the USA,
anywhere in the world where fluvalinate in any
formulation has been used for about 10 years for varroa
control resistance has developed. Since we are selecting
for the same traits in the population, I would expect the
curves to be very similar or the same where ever the
selection has occurred - this is the evidence that
mutation is not involved just selection of pre-existing
resistance. Apistan resistance occurred here in MN and
was documented first in one of the beekeeping outfits who
had varroa first and was therefore using apistan longest
( and yes I am very confident they were not using other
(illegal) treatments. Apistan selected for the resistance
at about the same rate as other formulations of
fluvalinate in other parts of the world. Don't get me
completely wrong there are many very good reasons to use
Apistan instead of those other formulations such as
contamination of honey and wax etc. but in terms of
selection!
For resistance I really see no difference.
FWIW
Blane White
MN Dept of Agriculture
Max Watkins
email@omitted.anti.spam
Hi Blane,
You are right, of course that in the States, the
widespread use of Apistan has probably been the strongest
selection pressure, as Klartan/other agro formulations
aren't used as frequently in beekeeping as in some other
countries (so I believe). This pyrethroid resistance was
bound to arise sooner or later, as there was only really
one control agent being used with a specific mode of
action. It doesn't matter what the formulation was, the
resistance to the active ingredient was inevitable in the
circumstances. If there had been other types of treatment
available, the selection for resistant strains would have
been at least slower, giving the industry longer use of
what tools it had.
It could be that resistance, caused directly by use of
Apistan, has appeared in Europe. It's possible. But in
most European countries, besides Apistan and Klartan
there are commonly other types of treatment used which
could have some retarding effect on the emergence of
resistant strains. The account that I gave earlier,
however, describes what happened in Italy and it looks
very much like the resistant populations emanated from
one central source. The uncontrolled dosage at that
source must have had an accelerating effect on resistance
emergence.
It may also be true that the dose-response curves for
pyrethroid-resistant mites in the USA are similar to
those in Europe - I don't know, as I've not seen any of
the US data but it would be interesting to compare. Do
the US mite population(s?) have the same characteristics
as the Western European mites? I agree that similar
traits should probably be selected for and the curves
indeed should look something like those we have generated
here. I just wonder if you'll see the resistance factor
of 400+ ? Maybe.
I'm not trying to set myself up as an expert and of
course, I may be entirely wrong. I do make mistakes -
let's see now, I remember I made one back in 1978....
Max
Peter Dillon
email@omitted.anti.spam-internet.fr
Max,
following this thread with much interest.
I am "surrounded" by beekeepers (99% of whom are
hobbyists, or in other words beekeepers who do not need
to gain a living from apicultural activities).
They are very much aware of the problem posed by V.j.
- many are now without hives due to its presence!
When I arrived here from England (at the time totally
free from V.j.), the first organisation I got into
contact with was the local group named Groupment
Departemental Defense Sanitaire (County Group Defending
Bee Health)- and when I asked what was the recognised
treatment against V.j. was instantly presented with some
"slim slivers of poplar wood that had been soaking in a
milky white liquid"
These were being sold I presume with the blessing of
the local County Vet. Office as the cheque that I HANDED
OVER WAS SIGNED TO DDGA. The whole idea of treatment was
chaotic - no controlled timing period for the area, no
recognised disposal of used strips - often they were left
laying around in apiaries.
As time proceeded, there was discussion relating to
changing the molecule due to resistance showing up - but
to my knowledge, this resistance was never tested for in
an organised manner, it was all hear say.
I had hives that when tested with Amitraze after being
treated with Apistan dropping several hundreds of mites,
others none at all - confusion reigned, at least in my
mind on what was really happening. As far as I am
concerned, treatment left in the hands of people who do
not either understand what they are doing or the
consequences of their actions is the best recipe for long
term disaster.
There appears to be a pretense that the Vet. services
are in control and know what is happening - they are not
and don't. The average beekeeper in my area will not
supply information and is distrusting of his fellow
beekeepers - just incase he/she finds out how much money
he/she is making/lossing.
We tried to set up a Development group for Beekeeping
in the area - failed due to apathy The different
beekeeping unions are at each others throats when ever
possible.
It is only when such disastrous situations as Gaucho
and Sunflowers arrive on the scene that sense prevails.
On the surface, everybody states that they are using the
recognised treatment for V.j., A.F.B.etc, but in
reality!!!, leaving those that do follow the rules to
suffer.
One old beekeeper came to the house and asked what I
THOUGHT ABOUT THAT NEW STUFF - Apifoss. I presumed he was
talking about COUMAPHOS ( even I am not sure about its
spelling). The vet. officer who was visiting put him
right by telling him not to use it but instead to use
Amitraze soaked onto jute strips. He realised the bloke
would never buy the official materials and considered it
better for all concerned that a treatment however it
arrived was better than none.
I gave up and fell into line - treat V.j. like the
rest and get good results whilst it lasts. Before I WAS
PAYING THE PREMIUM and not going to gain the extension in
time as most of the rest were happily making their 5%,10%
dips out of what ever was in fashion.
SO how are the trials relating to the pheromone that
will waylay females on their way to brood cells
going?
Yours truthfully
Peter
PS I do have excellent contacts with many serious
beekeepers throughout France. I HOPE THAT THE ABOVE
EXPLAINS THE DIFFERENCE BETWEEN WHAT SHOULD HAPPEN AND
WHAT REALLY HAPPENS.
Believing that the truth always come out one day!!
Max Watkins
email@omitted.anti.spam
Hi Peter,
I sympathise with your position in France. We've found
the exact same situation in many countries, including
France where quite a bit of our research is done. On the
one hand the "Authorities" set stringent rules for
registering a treatment - so stringent and expensive that
very few firms can get their product through to the user
and on the other hand you have what you described
perfectly - there is quite widespread disregard of the
rules even BY THE AUTHORITIES for this very
legislation.
We are asked constantly why we don't have the next
product(s) out yet. Registration alone (ignoring product
development time) can take 3 years. Vita is now just
coming to the point where we have registrations of new
products pending in many countries. Our APIGUARD thymol
gel is about to be widely registered so you can expect to
see that in France this year. APIGUARD is used for the
control of mites in honeybee colonies and will be 7 years
in reaching the market from the initial development
stages. Believe it or not, that is fast.
Our pheromone blend, PHEROVAR which interupts varroa
reproduction is about 18 months out of line. It's a
complicated blend and what we thought was the correct
constituency last year turned out to be not quite so. A
question of isomerism. We used the wrong isomer of three.
The blend is known but we have to go through all the
dosage, efficacy and bee tox tests again, hence the
delay. But it will come and news of it will be posted in
the bee press nearer the time.
Max
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